The simulation experiments demonstrated that during periods of unstable glycemic control, glycemic profiles with the same mean glucose might result in much different Hb A) has been used as an index of glycemic control in the management, clinical guidance, and clinical trials of diabetic patients for the past 35 years.In 2009, Hb A1c became a diagnostic test for diabetes [ tests repeated within a short time period or information from a patient that she or he experienced a substantial improvement in glycemic control a few weeks ago) to narrow this 15% uncertainty range.

validating hgb-35

Therefore, the glucose concentration in each culture was not constant but instead was changing, in a sawtooth-like manner, each day.

To minimize errors that were made during Hb A modeling, we interpolated these intraday changes of glucose concentration and used the interpolated values in the model.

We also sampled the cultures to measure Hb A (or our ability to properly remove nonviable cells and to detect viable cells), in the preliminary tests we used two methods in parallel to estimate the number of the viable cells in the cultures: a microscopic method with Bürker’s chamber and a cytometric method applying the Annexin V binding protocol.

In the preliminary tests, described above [ experiments using blood samples from the healthy volunteers.

Sp Hb was recorded using Masimo Radical-7 and compared with t Hb.

A total of three data sets were obtained for each patient.

Then we connected these profiles repeatedly to obtain the extrapolated 120-day course.

In the second method, the rescaled-to-plasma daily profiles were repeatedly copied to build the whole 120-day course, without any intermediate averaging (ID method).

Second, the hemolized erythrocytes were removed together with the old medium from a cell-culture dish, and then the fresh medium containing the desirable glucose concentration was added into the culture.

The difference between the glucose concentration at the beginning and at the end of each day was decreasing with time because the number of viable erythrocytes that were able to metabolize glucose was also decreasing.

The following procedure was used every day to sustain the presumed constant concentrations of glucose.