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Fetal bovine serum (FBS), 0.25 % trypsin containing EDTA and high glucose DMEM were obtained from Gibco (USA).Cell Counting Kit-8 (CCK-8) was purchased from Do Jin Do (Japan).This is true for metastatic diseases, wherein the average life expectancy is just three to 6 months.
Wound healing assay, transwell assay and spontaneous metastasis model were used to observe the migration activities.
Real-time PCR, immunofluorescence, western blot analysis and immunohistochemistry methods were used to observe the expression of genes or proteins. Real-time PCR and immuno-fluorescence analyses of epithelial-to-mesenchymal transition (EMT) markers were compared between control group and ORI group.
ORI was purchased from the Yuanye Biological Institutions (Shanghai, China).
Its purity was measured by HPLC and determined to be 99 %.
Less than 20 % of patients with pancreatic cancer are subjected to surgical removal, whereas other patients are typically treated with chemotherapy or chemotherapy with radiation.
Pancreatic cancer is one of the few cancers in which survival has not improved substantially in 40 years.
Oridonin (ORI) can inhibit proliferation and migration in various types of cancer cell lines. We investigated the migration inhibitory effect of ORI on human pancreatic cancer SW1990 cells and dissected the possible molecular mechanism(s).
CCK-8 assay was used to observe the cell viability.
ORI can inhibit pancreatic cancer cell SW1990 migration and EMT by down-regulating Wnt/β-catenin signal transduction in vitro and in vivo.
Therefore, it can be potentially and effectively used in the clinical management of pancreatic cancer.
These patients have worse prognoses than those who demonstrate less evidence of EMT.